ALAD antibody (Middle Region)

Catalog No. ABIN2776922

This Rabbit Polyclonal antibody specifically detects ALAD in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Saccharomyces cerevisiae.

Quick Overview for ALAD antibody (Middle Region) (ABIN2776922)

Target: ALAD (Aminolevulinate Dehydratase (ALAD))

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALAD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ALAD Antibody

Binding Specificity: Middle Region

Purpose: ALAD Antibody - middle region

Sequence: SVMSYSAKFA SCFYGPFRDA AKSSPAFGDR RCYQLPPGAR GLALRAVDRD

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%, Yeast: 86%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against ALAD. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ALAD

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8094533

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ALAD

Target: ALAD (Aminolevulinate Dehydratase (ALAD))

Alternative Name: ALAD

Background:

Background Information: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 Molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria.The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 Molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternatively spliced transcript variants encoding different isoforms have been identified.

Gene Name: Aminolevulinate dehydratase

Alternative Symbols: PBGS, ALADH

Protein Name: Delta-aminolevulinic acid dehydratase RuleBase RU000515

Molecular Weight: 39kDa

Gene ID: 210

NCBI Accession: NP_001003945

UniProt: Q6ZMU0