Keratin 20 (KRT20) antibody
| Antigen |
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| Synonyms |
K20, CD20, CK20, KRT21, MGC35423, 9030623C06Rik, Krt21, KRT20, MGC152269 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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 Alternatives Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
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Conjugate
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FITC (4), Biotin (3), HRP (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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Western Blotting (WB) (67), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (60), Immunohistochemistry (IHC) (32), ELISA (23), Immunofluorescence (IF) (22), Immunoprecipitation (IP) (10), Immunohistochemistry (Fixed) (IHC (fx)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Flow Cytometry (FACS) (2), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN310358 |
| Quantity |
0.1 mg |
| Price |
251.90 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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Cytokeratin 20
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Gene ID
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KRT20
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UniProt
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P35900
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Immunogen
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The immunogen for anti-KRT20 antibody: synthetic peptide directed towards the middle region of human KRT20
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Sequence
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DDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLG NTVNVEVDAA
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Cross-Reactivity
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Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
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Format
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Lyophilized
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Reconstitution
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Add 100 µl of distilled water. Final anti-KRT20 antibody concentration is 1 mg/ml.
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Description
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KRT20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa.The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
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Characteristics
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Antigen Size: 424 amino acids
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Molecular Weight
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48kDa
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Application Notes
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WB Suggested Anti-KRT20 Antibody Titration: 1.25ug/ml
Positive Control: DLD1 cell lysate.
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Purification
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Protein A purified
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Buffer
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PBS
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Storage (Long Term)
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For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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Storage (Short Term)
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Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
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Research Area
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Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer
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Restrictions
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For Research Use only
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Product
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Wong, Cheung, Ma et al.: "Isolated tumor cells and circulating CK20 mRNA in pN0 colorectal cancer patients." in: International journal of surgical pathology, Vol. 16, Issue 2, pp. 119-26, 2008 (PubMed).
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Alternatives for antigen "Keratin 20 (KRT20)", type "Antibodies"
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Hosts
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Mouse (59), Rabbit (48), Guinea Pig (2)
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Reactivities
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Human (100), Rat (Rattus) (28), Mouse (Murine) (25), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)
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Applications
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Western Blotting (WB) (67), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (60), Immunohistochemistry (IHC) (32), ELISA (23), Immunofluorescence (IF) (22), Immunoprecipitation (IP) (10), Immunohistochemistry (Fixed) (IHC (fx)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Flow Cytometry (FACS) (2), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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FITC (4), Biotin (3), HRP (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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C-Term (4), Internal Region (1)
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2013 BioMed Central Research Awards
