Ring Finger Protein 128 (RNF128) antibody

Antigen: Ring Finger Protein 128 (RNF128)

Synonyms: GRAIL, FLJ23516

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Dog (Canine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN310450

Additional Information

Alternative name: RNF128

Gene ID: RNF128

UniProt: Q8TEB7

Immunogen: The immunogen for anti-RNF128 antibody: synthetic peptide directed towards the C terminal of human RNF128

Sequence: LEEHVQSTNESLQLVNHEANSVAVDVIPHVDNPTFEEDET PNQETAVREI

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 100 µl of distilled water. Final anti-RNF128 antibody concentration is 1 mg/ml.

Description: RNF128 is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of RNF128 in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of RNF128 was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype.The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Characteristics: Antigen Size: 428 amino acids

Molecular Weight: 47kDa

Application Details

Application Notes: WB Suggested Anti-RNF128 Antibody Titration: 1.25ug/ml
Positive Control: HepG2 cell lysate.

Purification: Protein A purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Research Area: Proteolysis / Ubiquitin

Restrictions: For Research Use only

Images

WB Suggested Anti-RNF128 Antibody Titration: 1.25ug/ml
Positive Control: HepG2 cell lysate

Publications

Product: Soares, Seroogy, Skrenta et al.: "Two isoforms of otubain 1 regulate T cell anergy via GRAIL." in: Nature immunology, Vol. 5, Issue 1, pp. 45-54, 2003 (PubMed).