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Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2) antibody

Antigen

Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2)

Synonyms KAT4, KATIV, mitAAT, FLJ40994, got2, GOT2, DKFZp459L1636
Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Zebrafish, Pig (Porcine)

Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB)
1 reference available
Catalog no. ABIN310472
Quantity 0.1 mg  (Variants)
Price 251.90 $   Plus shipping costs $45.00
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Additional Information

Alternative name GOT2
Gene ID GOT2
UniProt P00505
Immunogen The immunogen for anti-GOT2 antibody: synthetic peptide directed towards the N terminal of human GOT2
Sequence PPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPS VRKAEAQIAA
Cross-Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
Format Lyophilized
Reconstitution Add 100 µl of distilled water. Final anti-GOT2 antibody concentration is 1 mg/ml.
Description Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Characteristics Antigen Size: 430 amino acids
Molecular Weight 45kDa

Application Details

Application Notes WB Suggested Anti-GOT2 Antibody Titration: 1.0ug/ml
ELISA Titer: 1:312500
Positive Control: 293T cell lysate.
Purification Protein A purified
Buffer PBS
Storage (Long Term) For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Storage (Short Term) Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
Research Area Cancer, Metabolism, Amino Acids
Restrictions For Research Use only

Publications

Product Suzuki, Yoshitomo-Nakagawa, Maruyama et al.: "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library." in: Gene, Vol. 200, Issue 1-2, pp. 149-56, 1997 (PubMed).

Alternatives

Alternatives for antigen "Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2)", type "Antibodies"
Hosts Rabbit (42), Mouse (25), Sheep (9), Goat (8), Rat (1)
Reactivities Human (64), Rat (Rattus) (38), Mouse (Murine) (35), Pig (Porcine) (6), Monkey (3), Chicken (2), Cow (Bovine) (2), Cat (Feline) (1), Dog (Canine) (1), Primate (1), Xenopus laevis (1), Zebrafish (1)
Applications Western Blotting (WB) (52), ELISA (43), Immunofluorescence (IF) (26), Immunohistochemistry (IHC) (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Enzyme Immunoassay (EIA) (9), Immunoprecipitation (IP) (9), Immunocytochemistry (ICC) (6), Dot Blot (Dot) (4), Radioimmunoassay (RIA) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (DB) (2), Flow Cytometry (FACS) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (6), HRP (3), FITC (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes AA 295-306 (3), AA 360-373 (3), N-Term (3), AA 25-430 (2), C-Term (1)