NDUFB5 antibody (Middle Region)

Catalog No. ABIN310669

This Rabbit Polyclonal antibody specifically detects NDUFB5 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for NDUFB5 antibody (Middle Region) (ABIN310669)

Target: NDUFB5 (NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 5, 16kDa (NDUFB5))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDUFB5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NDUFB5 Antibody

Binding Specificity: Middle Region

Sequence: KLLRFYIALT GIPVAIFITL VNVFIGQAEL AEIPEGYVPE HWEYYKHPIS

Predicted Reactivity: Cow: 93%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 86%, Rat: 86%, Zebrafish: 83%

Characteristics: This is a rabbit polyclonal antibody against NDUFB5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NDUFB5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 189 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NDUFB5 antibody (ABIN310669)

Ton, Hwang, Dempsey, Liew: "Identification and primary structure of five human NADH-ubiquinone oxidoreductase subunits." in: Biochemical and biophysical research communications, Vol. 241, Issue 2, pp. 589-94, (1998) (PubMed).

Target Details for NDUFB5

Target: NDUFB5 (NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 5, 16kDa (NDUFB5))

Alternative Name: NDUFB5

Background: NDUFB5 is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Alias Symbols: CI-SGDH, DKFZp686N02262, FLJ30597, MGC111204, MGC12314, SGDH, CISGDH
Protein Interaction Partner: UBC, NDUFS4, NDUFS1, NDUFB9, MPV17, ICT1, DLD, ATP5F1, NDUFS7, MARCKSL1, MRPS26, MSLN, LAMTOR3, SLC25A3,
Protein Size: 189

Molecular Weight: 17 kDa

Gene ID: 4711

NCBI Accession: NM_002492, NP_002483

UniProt: O43674