ECHS1 antibody (Middle Region)

Catalog No. ABIN310715

The Rabbit Polyclonal anti-ECHS1 antibody is suitable to detect ECHS1 in samples from Human, Mouse, Rat, Dog, Cow, Rabbit, Zebrafish (Danio rerio), Guinea Pig and Horse. It has been validated for WB.

Quick Overview for ECHS1 antibody (Middle Region) (ABIN310715)

Target: ECHS1 (Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECHS1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ECHS1 Antibody

Binding Specificity: Middle Region

Sequence: RAVGKSLAME MVLTGDRISA QDAKQAGLVS KICPVETLVE EAIQCAEKIA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against ECHS1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ECHS1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 290 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ECHS1

Target: ECHS1 (Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1))

Alternative Name: ECHS1

Background: ECHS1 functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. ECHS1 is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix.The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature.
Alias Symbols: SCEH
Protein Interaction Partner: SUMO2, UBC, MDM2, GINS3, SMEK2, CNDP2, DCPS, ABHD14A, YWHAQ, SLC9A3R1, USP14, YWHAZ, VCL, UBE2V1, TGM2, RAP1GDS1, PFAS, HSPA9, GSS, CAPN1, CLDN12, UBA1, PPM1B, SERPINA1, ECHS1, CYP2E1, GRB2, UCHL5, UBA5, DDA1, CBWD2, EPB41,
Protein Size: 290

Molecular Weight: 28 kDa

Gene ID: 1892

NCBI Accession: NM_004092, NP_004083

UniProt: P30084

Pathways: Monocarboxylic Acid Catabolic Process