phosphopantothenoylcysteine Decarboxylase (PPCDC) (N-Term) antibody

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Antigen
Epitope
N-Term
(19), (15), (3), (2), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(16), (14), (4), (1), (1), (1)
Host
Rabbit
(26), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(24), (16), (3), (2), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Synthetic peptide directed towards the N terminal of human PPCDC
Sequence VTTERAKHFY SPQDIPVTLY SDADEWEIWK SRSDPVLHID LRRWADLLLV
Predicted Reactivity Dog : 100 %, Guinea pig : 90 %, Rat : 90 %, Bovine : 80 %, Chicken : 80 %, Horse : 80 %, Pig : 80 %
Characteristics This is a rabbit polyclonal antibody against PPCDC. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name PPCDC (PPCDC Antibody Abstract)
Background Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC, one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine.
Molecular Weight 22 kDa
Gene ID 60490
NCBI Accession NP_068595, NM_021823
UniProt Q96CD2
Research Area Signaling, Metabolism
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 204 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-phosphopantothenoylcysteine Decarboxylase (PPCDC) (N-Term) antibody (ABIN310805) WB Suggested Anti-PPCDC Antibody Titration: 2.5ug/ml
Positive Control: <...
Background publications Daugherty, Polanuyer, Farrell et al.: "Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics." in: The Journal of biological chemistry, Vol. 277, Issue 24, pp. 21431-9, 2002 (PubMed).

Catalog No. ABIN310805
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