Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) antibody

Antigen: Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1)

Synonyms: PDHA, PHE1A, PDHCE1A, Pdha-1, MGC94854, MGC114215, pdha, phe1a, pdhce1a, DKFZp459C032

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN310981

Additional Information

Alternative name: PDHA1

Description: The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT, MIM 608770) (E2, EC 2.3.1.12), and dihydrolipoyl dehydrogenase (DLD, MIM 238331) (E3, EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. Keywords: PDHA1, NM_000284, NP_000275, P08559

Application Details

Application Notes: ELISA titre: 1:312500 . Western Blotting: Suggested dilution at 1.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.

Restrictions: For Research Use only

Images

anti-Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) antibody

Publications

Publications: Boichard, Venet, Naas et al.: "Two silent substitutions in the PDHA1 gene cause exon 5 skipping by disruption of a putative exonic splicing enhancer." in: Molecular genetics and metabolism, Vol. 93, Issue 3, pp. 323-30, 2008 (PubMed).