RFT1 antibody (N-Term)

Catalog No. ABIN311263

This Rabbit Polyclonal antibody specifically detects RFT1 in WB. It exhibits reactivity toward Human, Rat, Dog, Mouse, Cow, Guinea Pig, Horse and Rabbit.

Quick Overview for RFT1 antibody (N-Term) (ABIN311263)

Target: RFT1 (RFT1 Homolog (RFT1))

Reactivity: Human, Rat, Dog, Mouse, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RFT1 Antibody

Binding Specificity: N-Term

Purpose: RFT1 Antibody - N-terminal region

Sequence: GTQRDWSQTL NLLWLTVPLG VFWSLFLGWI WLQLLEVPDP NVVPHYATGV

Predicted Reactivity: Cow: 92%, Dog: 100%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 85%, Rabbit: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against RFT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RFT1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8110451

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RFT1

Target: RFT1 (RFT1 Homolog (RFT1))

Alternative Name: RFT1

Background:

Background Information: N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a ! !Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation.N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation (Helenius et al., 2002 [PubMed 11807558]).[supplied by OMIM].

Gene Name: RFT1 homolog (S. cerevisiae)

Alternative Symbols: CDG1N

Protein Name: Protein RFT1 homolog

Molecular Weight: 60kDa

Gene ID: 91869

NCBI Accession: NP_443091

UniProt: Q96AA3