CYP1B1 antibody (Middle Region)

Catalog No. ABIN311461

Product Details anti-CYP1B1 Antibody

Target: CYP1B1 (Cytochrome P450, Family 1, Subfamily B, Polypeptide 1 (CYP1B1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP1B1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AVANVMSAVC FGCRYSHDDP EFRELLSHNE EFGRTVGAGS LVDVMPWLQY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CYP1B1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CYP1B1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 543 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CYP1B1 antibody (ABIN311461)

Staršíchová, Hrubá, Slabáková, Pernicová, Procházková, P?n?íková, Seda, Kabátková, Vondrá?ek, Kozubík, Machala, Sou?ek: "TGF-?1 signaling plays a dominant role in the crosstalk between TGF-?1 and the aryl hydrocarbon receptor ligand in prostate epithelial cells." in: Cellular signalling, Vol. 24, Issue 8, pp. 1665-76, (2012) (PubMed).

Wang, Hawkins, Miller: "Aryl hydrocarbon receptor-mediated up-regulation of ATP-driven xenobiotic efflux transporters at the blood-brain barrier." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 2, pp. 644-52, (2011) (PubMed).

Target Details for CYP1B1

Target: CYP1B1 (Cytochrome P450, Family 1, Subfamily B, Polypeptide 1 (CYP1B1))

Alternative Name: CYP1B1 (CYP1B1 Products)

Background: CYP1B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP1B1 localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma, therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid.This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma, therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CP1B, GLC3A, P4501B1, CYPIB1
Protein Interaction Partner: UBC, SAE1,
Protein Size: 543

Molecular Weight: 61 kDa

Gene ID: 1545

NCBI Accession: NM_000104, NP_000095

UniProt: Q16678

Pathways: Steroid Hormone Biosynthesis