CYP11A1 antibody (Middle Region)

Catalog No. ABIN311472

The Rabbit Polyclonal anti-CYP11A1 antibody (ABIN311472) specifically detects CYP11A1 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for CYP11A1 antibody (Middle Region) (ABIN311472)

Target: CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP11A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CYP11A1 Antibody

Binding Specificity: Middle Region

Purpose: CYP11A1 Antibody - middle region

Sequence: LRQKGSVHHD YRGILYRLLG DSKMSFEDIK ANVTEMLAGG VDTTSMTLQW

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against CYP11A1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CYP11A1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098354

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CYP11A1

Target: CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))

Alternative Name: CYP11A1

Background:

Background Information: CYP11A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP11A1 localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide.

Gene Name: Cytochrome P450, family 11, subfamily A, polypeptide 1

Alternative Symbols: CYP11A, CYPXIA1, P450SCC

Protein Name: Cholesterol side-chain cleavage enzyme, mitochondrial

Molecular Weight: 60 kDa

Gene ID: 1583

NCBI Accession: NP_000772

UniProt: P05108

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Cellular Response to Molecule of Bacterial Origin