ABCB1 antibody

Catalog No. ABIN317068

Product Details anti-ABCB1 Antibody

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This ABCB1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody specifically recognises an extracellular conformational epitope of CD243, also known as MDR1 (multidrug resistance protein 1) and Pgp (Pglycoprotein), a transmembrane protein and member of the ABC transporter (ATPbinding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds. Clone UIC2 can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell. Clone UIC2 does not cross-react with mitochondrial pyruvate carboxylase. We recommend the use of AM05632LE-N for Functional Studies.

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Species reactivity (tested):Human, Primate.

Characteristics: Synonyms: Multidrug resistance protein 1, P-glycoprotein 1, ABCB1, PGY1, ATP-binding cassettesub-family B member 1

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA.

Clone: UIC2

Isotype: IgG2a

Application Details

Application Notes: Immunoprecipitation. Immunofluorescence. Flow Cytometry: Use 10 μL of neat MDR1/CD243 antibody to label 1x10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200, This product may benefit from antigenretrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled bytrained staff only.

Expiry Date: 12 months

Target Details for ABCB1

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Alternative Name: CD243 / MDR1 (ABCB1 Products)

Background: CD243 is also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein), a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds. CD243 is expressed at low levels in the cell membrane of peripheral blood leucocytes, and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. CD243 mediates resistance to many chemotherapeutic agents used for tumour suppression and is therefore of special interest to oncologists. Clone UIC2 is a strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs.Synonyms: ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, PGY1

Gene ID: 5243

UniProt: P08183