HNF4A antibody

Catalog No. ABIN317780

There are 2+ publications for this product available. The Rabbit Polyclonal anti-HNF4A antibody is suitable to detect HNF4A in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for HNF4A antibody (ABIN317780)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HNF4A Antibody

Specificity: This antibody detects endogenous levels of HNF4α protein.(region surrounding Lys298)

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-HNF4A antibody (ABIN317780)

Hu, Ma, Huang, Mao, Yang, Zhao, Li, Qiu, Yang, Zheng, Wang: "Dihydrocapsaicin Attenuates Plaque Formation through a PPAR?/LXR? Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet." in: PLoS ONE, Vol. 8, Issue 6, pp. e66876, (2013) (PubMed).

He, Xiao, Zhang, Ma, Li, Yang: "Detection and functional annotation of misregulated microRNAs in the brain of the Ts65Dn mouse model of Down syndrome." in: Chinese medical journal, Vol. 126, Issue 1, pp. 108-13, (2013) (PubMed).

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4 alpha / TCF14

Background: HNF-1 (α and β), HNF-3 (α, β and γ), HNF-4 (α and γ), and HNF-6 compose, in part, a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3α, HNF-3β and HNF-3γ regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3α and HNF-3β have also been shown to be involved in gastrulation events such as body axis formation. HNF-4α and HNF-4γ have been shown to be important for early embryo development. HNF-4α is expressed in liver, kidney, pancreas, small intestine, testis and colon, and HNF-4γ is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3β, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and α-1 antitrypsin.Synonyms: HNF4, HNF4A, Hepatocyte nuclear factor 4-alpha, NR2A1, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, Transcription factor HNF-4

Molecular Weight: 52 kDa

Gene ID: 3172

NCBI Accession: NP_000448

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process