HNRNPK antibody

Catalog No. ABIN317794

This Rabbit Polyclonal antibody specifically detects HNRNPK in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HNRNPK antibody (ABIN317794)

Target: HNRNPK (Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HNRNPK Antibody

Specificity: HnRNP K antibody detects endogenous levels of hnRNP K protein.(region surrounding Tyr280)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 1000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HNRNPK

Target: HNRNPK (Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))

Alternative Name: HnRNP-K / HNRNPK

Background: Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65 kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.Synonyms: HNRPK, Heterogeneous nuclear ribonucleoprotein K, TUNP, Transformation up-regulated nuclear protein

Molecular Weight: approx. 56 kDa

Gene ID: 3190

NCBI Accession: NP_002131

UniProt: P61978