MARK1/2/3/4 antibody

Catalog No. ABIN317798

The Rabbit Polyclonal anti-MARK1/2/3/4 antibody is suitable to detect MARK1/2/3/4 in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for MARK1/2/3/4 antibody (ABIN317798)

Target: MARK1/2/3/4 (MAP/microtubule Affinity-Regulating Kinase 1/2/3/4 (MARK1/2/3/4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARK1/2/3/4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MARK1/2/3/4 Antibody

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MARK1/2/3/4

Target: MARK1/2/3/4 (MAP/microtubule Affinity-Regulating Kinase 1/2/3/4 (MARK1/2/3/4))

Alternative Name: MARK1/2/3/4

Background: MARK1 (MAP/microtubule affinity-regulating kinase 1) belongs to the MARK family of serine/threonine kinases. MARK family protein kinases phosphorylate microtubule-associated proteins and may play a role in cytoskeletal stability. MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1 called MARK2. MARK3 was originally identified as a marker that was induced by treatment with DNA damaging agents, and loss of MARK3 was found with carcinogenesis in the pancreas. MARK4 contains an N terminal serine/threonine kinase domain, a central ubiquitin associated domain, and a C terminal KA1 associated kinase domain. RT PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells.Synonyms: MAP/Microtubule Affinity-Regulating Kinase, MARK

Molecular Weight: approx. 92 kDa