EIF3E antibody

Catalog No. ABIN317905

This Rabbit Polyclonal antibody specifically detects EIF3E in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for EIF3E antibody (ABIN317905)

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF3E Antibody

Specificity: EIF3ε antibody detects endogenous levels of eIF3ε protein.(region surrounding Val116)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EIF3E

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Alternative Name: EIF3E / EIF3S6

Background: Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Eukaryotic initiation factor 3 (eIF3) is the largest family of eIFs and consists of at least 12 unique subunits in mammals. eIFε, also known as eIF p47, binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA and associates with the complex p170-eIF3.Synonyms: Eukaryotic translation initiation factor 3 subunit 6, Eukaryotic translation initiation factor 3 subunit E, INT6, Viral integration site protein INT-6 homolog, eIF-3 p48

Molecular Weight: approx. 38 kDa

Gene ID: 3646

NCBI Accession: NP_001559

UniProt: P60228

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C