EIF3E antibody (N-Term)

Catalog No. ABIN2783259

The Rabbit Polyclonal anti-EIF3E antibody is suitable to detect EIF3E in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse and Rabbit. It has been validated for WB and IHC.

Quick Overview for EIF3E antibody (N-Term) (ABIN2783259)

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EIF3E Antibody

Binding Specificity: N-Term

Purpose: EIF3E Antibody - N-terminal region

Sequence: ELLQGKLDLL SDTNMVDFAM DVYKNLYSDD IPHALREKRT TVVAQLKQLQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against EIF3E. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EIF3E

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48175-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF3E

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Alternative Name: EIF3E

Background:

Targeted Subunit: E

Background Information: EIF3E belongs to the eIF-3 subunit E family.It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. EIF3E is required for nonsense-mediated mRNA decay (NMD), It may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. The protein may interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

Gene Name: Eukaryotic translation initiation factor 3, subunit E

Alternative Symbols: INT6, EIF3S6, EIF3-P48, eIF3-p46

Protein Name: Eukaryotic translation initiation factor 3 subunit E

Molecular Weight: 52kDa

Gene ID: 3646

NCBI Accession: NP_001559

UniProt: P60228

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C