FUBP3 antibody

Catalog No. ABIN317906

The Rabbit Polyclonal anti-FUBP3 antibody is suitable to detect FUBP3 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for FUBP3 antibody (ABIN317906)

Target: FUBP3 (Far Upstream Element (FUSE) Binding Protein 3 (FUBP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FUBP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FUBP3 Antibody

Specificity: This antibody detects endogenous levels of FBP3 protein.(region surrounding Arg241)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FUBP3

Target: FUBP3 (Far Upstream Element (FUSE) Binding Protein 3 (FUBP3))

Alternative Name: FUBP3

Background: Activation of FUSE, the far-upstream element, is required for the proper expression of the mammalian gene c-Myc in undifferentiated cells. The binding of FBP (FUSE-binding protein) to FUSE is necessary for c-Myc expression, indicating that FBP functions as a growth-dependent regulator of c-Myc expression. Isolated from proliferating HL60 cells, FBP, FBP2, and FBP3 comprise a family of single-stranded DNA-binding proteins that specifically bind to FUSE elements. The FBP transcription factors share a conserved central DNA-binding domain and show significant homology in their carboxylterminal activation domains. Expression of FBP is detected in undifferentiated cells and is substantially decreased following cellular differentiation.Synonyms: FBP3, FUSE-binding protein 3, Far upstream element-binding protein 3

Molecular Weight: approx. 60 kDa

Gene ID: 8939

NCBI Accession: NP_003925

UniProt: Q96I24