GNGT1 antibody

Catalog No. ABIN317913

The Rabbit Polyclonal anti-GNGT1 antibody (ABIN317913) specifically detects GNGT1 in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for GNGT1 antibody (ABIN317913)

Target: GNGT1 (G Protein gamma Transducing Activity Polypeptide 1 (GNGT1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNGT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GNGT1 Antibody

Specificity: Gα t1 antibody detects endogenous levels of Gα t1 protein.(region surrounding Met104)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GNGT1

Target: GNGT1 (G Protein gamma Transducing Activity Polypeptide 1 (GNGT1))

Alternative Name: G Protein gamma T1

Background: Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein α, β and γ polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their α subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Gα subunits have been identified, these include Gs, Gi, Gq and Gα 12/13. The Gi class comprises all the known α subunits that are susceptible to pertussis toxin modifications, including Gα i-1, Gα i-2, Gα i-3, Gα o, Gα t1, Gα t2, Gα z and Gα gust. In the well characterized visual system, photorhodopsin catalyzes the exchange of guanine nucleotides bound to the visual transducin Gα subunits (Gα t1 in rod cells and Gα t2 in cone cells).Synonyms: GNGT1, Guanine nucleotide-binding protein G T subunit gamma-T1, Transducin gamma chain

Molecular Weight: approx. 36 kDa

Gene ID: 2792

NCBI Accession: NP_068774

UniProt: P63211

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction