SLC16A3 antibody

Catalog No. ABIN317918

The Rabbit Polyclonal anti-SLC16A3 antibody is suitable to detect SLC16A3 in samples from Human, Rat and Mouse. It has been validated for WB.

Quick Overview for SLC16A3 antibody (ABIN317918)

Target: SLC16A3 (Solute Carrier Family 16 (Monocarboxylic Acid Transporters), Member 3 (SLC16A3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC16A3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SLC16A3 Antibody

Specificity: This antibody detects endogenous levels of MCT4 protein.(region surrounding Phe265)

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/40000-1/60000. Western Blot: 1/500-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 with 0.05 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SLC16A3

Target: SLC16A3 (Solute Carrier Family 16 (Monocarboxylic Acid Transporters), Member 3 (SLC16A3))

Alternative Name: SLC16A3

Background: Monocarboxylates, such as lactate and pyruvate, play an integral role in cellular metabolism. Lactic acid is produced in large quantities as a result of glycolysis, which provides the majority of ATP to cells under normal physiological conditions. However, accumulation of lactic acid leads to a decrease in intracellular pH and cessation of glycolysis. In order for glycolysis to continue at a high rate, lactic acid must be transported out of the cell. This transport process is carried out by a family of monocarboxylate transporters (MCTs),which function as proton symports and are stereoselective for L-lactate.The MCT family consists of at least eight members, MCT1-8, which contain between 10-12 transmembrane-helical (TM) domains, with the amino and carboxy termini located in the cytoplasm. MCT1 is widely expressed and is the major form of MCTs in tumor cells and erythrocytes. MCT2 is highly expressed in liver and testis, while MCT3 and MCT4 are predominantly expressed in skeletal muscle.Synonyms: MCT 3, MCT 4, MCT3, MCT4, Monocarboxylate transporter 3, Monocarboxylate transporter 4, Solute carrier family 16 member 3

Molecular Weight: approx. 49 kDa

Gene ID: 9123

NCBI Accession: NP_001035887

UniProt: O15427

Pathways: Warburg Effect