HRK antibody (Middle Region)

Catalog No. ABIN318751

The Rabbit Polyclonal anti-HRK antibody (ABIN318751) specifically detects HRK in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for HRK antibody (Middle Region) (ABIN318751)

Target: HRK (Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain) (HRK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HRK Antibody

Binding Specificity: Middle Region

Specificity: This antibody detects Harakiri (HRK), also known as DP5.

Purification: Purified

Immunogen: 15 amino acid peptide from near the centre of human HRK (NP_003797).

Isotype: IgG

Application Details

Application Notes: Western Blot: 2 - 5g/mL, detects a band of approximately 13 kDa in mouse pancreas tissuelysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for HRK

Target: HRK (Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain) (HRK))

Alternative Name: Harakiri / HRK

Background: HRK is a BH3 only protein of the Bcl-2 family, which promotes Bax dependent apoptosis. HRK regulates apoptosis through its interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL, and in conjunction to the mitochondrial pore forming protein p32. HRK is thought to have a specific role in initiating neuronal and haematopoietic cell apoptosis, in particular to growth factor deprivation. Aberrant methylation of HRK is frequently detected in primary colorectal cancers. Despite its predicted molecular weight, HRK often migrates at 12-15kD.Synonyms: Activator of apoptosis harakiri, BH3-interacting domain-containing protein 3, BID3, Neuronal death protein DP5

Gene ID: 8739

NCBI Accession: NP_003797

UniProt: O00198