Quick Overview for HDAC4/HDAC5/HDAC9 antibody (pSer220, pSer246, pSer259) (ABIN319280)
Target
HDAC4/HDAC5/HDAC9
(HDAC4,HDAC5,HDAC9)
Reactivity
Human, Mouse
Host
Rabbit
Clonality
Polyclonal
Conjugate
This HDAC4/HDAC5/HDAC9 antibody is un-conjugated
Application
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Binding Specificity
pSer220, pSer246, pSer259
Purification
Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human HDAC4/HDAC5/HDAC9 around the phosphorylation site of Serine 246/259/220 (T-A-SP-EP).
Western Blot (1/500-1/1000). Immunohistochemistry (1/50-1/100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.