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HDAC4/HDAC5/HDAC9 antibody (pSer220, pSer246, pSer259)

This Rabbit Polyclonal antibody specifically detects HDAC4/HDAC5/HDAC9 in WB and IHC (p). It exhibits reactivity toward Human and Mouse.
Catalog No. ABIN319280

Quick Overview for HDAC4/HDAC5/HDAC9 antibody (pSer220, pSer246, pSer259) (ABIN319280)

Target

HDAC4/HDAC5/HDAC9 (HDAC4,HDAC5,HDAC9)

Reactivity

  • 22
  • 12
  • 1
Human, Mouse

Host

  • 22
Rabbit

Clonality

  • 22
Polyclonal

Conjugate

  • 8
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
This HDAC4/HDAC5/HDAC9 antibody is un-conjugated

Application

  • 8
  • 6
  • 4
  • 2
  • 2
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

    • 11
    • 10
    • 9
    • 2
    • 1
    • 1
    • 1
    pSer220, pSer246, pSer259

    Purification

    Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

    Immunogen

    The antiserum was produced against synthesized phosphopeptide derived from human HDAC4/HDAC5/HDAC9 around the phosphorylation site of Serine 246/259/220 (T-A-SP-EP).
  • Application Notes

    Western Blot (1/500-1/1000). Immunohistochemistry (1/50-1/100).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Buffer

    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Store the antibody (in aliquots) at-20 °C.
  • Target

    HDAC4/HDAC5/HDAC9 (HDAC4,HDAC5,HDAC9)

    Background

    Synonyms: HDAC-4, HDAC-5, HDAC-9, Histone Deacetylase
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