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CD33 antibody

CD33 Reactivity: Human FACS, WB, IP Host: Mouse Monoclonal WM53 unconjugated

Catalog No. ABIN319734

Datasheet as PDF

Target See all CD33 Antibodies

CD33
Reactivity
168

35

15

13

7

6

3

1
Human
Host
100

74
Mouse
Compatible Secondaries
Clonality
95

78

1
Monoclonal
Conjugate
58

16

14

13

10

6

5

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD33 antibody is un-conjugated
Application
107

104

44

35

26

26

23

22

16

13

11

9

6

4

4

2

2

2

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Specificity

Recognizes the CD33 cell surface glycoprotein, a 67 kDa molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias.

Cross-Reactivity (Details)

Species reactivity (tested):Human.

Purification

Affinity Chromatography on Protein G.

Immunogen

Human AML cells

Clone

WM53

Isotype

IgG1

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anti-CD33 (CD33) (AA 101-200) antibody
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anti-CD33 (CD33) (AA 15-237) antibody
CD33 Reactivity: Human FACS, WB, ELISA, IHC Host: Mouse Monoclonal 2C6B7 unconjugated

anti-CD33 (CD33) (AA 18-240) antibody
CD33 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 49-259) antibody
CD33 Reactivity: Human WB, IF, IHC Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 261-364) antibody
CD33 Reactivity: Human FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 1-364) antibody
CD33 Reactivity: Human WB, ELISA, RNAi Host: Mouse Monoclonal 2D12-G4 unconjugated

anti-CD33 (CD33) (AA 18-269) antibody
CD33 Reactivity: Human FACS, ELISA Host: Rabbit Monoclonal DM77 unconjugated

anti-CD33 (CD33) antibody
CD33 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, CyTOF Host: Mouse Monoclonal WM53 unconjugated

anti-CD33 (CD33) antibody
CD33 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, Func, CyTOF Host: Mouse Monoclonal WM53 unconjugated

anti-CD33 (CD33) antibody (Biotin)
CD33 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal HIM3-4 Biotin

Application Notes

Flow Cytometry: Use 10 μL of 1/10-1/50 diluted antibody to label 10^6 cells. Immunoprecipitation. Immunohistochemistry on Frozen Sections (1/50-1/100): Overnight incubation recommended. Positive control tissue: lymphatic tissue. Not suitable on Paraffin Embedded Sections. Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS, pH 7.4, 0.09 % Sodium Azide

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

CD33

Alternative Name

CD33 / SIGLEC3 (CD33 Products)

Synonyms

SIGLEC-3 antibody, SIGLEC3 antibody, p67 antibody, Siglec-3 antibody, gp67 antibody, CD33 molecule antibody, CD33 antigen antibody, CD33 antibody, Cd33 antibody

Background

CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67

Gene ID

945

NCBI Accession

NP_001763

UniProt

P20138