CD33 antibody (AA 18-269)

Catalog No. ABIN7092878

Product Details anti-CD33 Antibody

Target: CD33

Binding Specificity: AA 18-269

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD33 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA

Purification: Purified from cell culture supernatant by affinity chromatography

Immunogen: Recombinant human CD33 (Asp18-His269) (ABIN6961110) produced by using human HEK293 cells

Clone: DM77

Isotype: IgG

Application Details

Application Notes: ELISA 1/5000-10000,Flow Cyt  1/100

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with deionized water

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.,0.1 % Procline 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details for CD33

Target: CD33

Alternative Name: CD33 (CD33 Products)

Synonyms: SIGLEC-3 antibody, SIGLEC3 antibody, p67 antibody, Siglec-3 antibody, gp67 antibody, CD33 molecule antibody, CD33 antigen antibody, CD33 antibody, Cd33 antibody

Background: Synonyms:CD33,SIGLEC3,gp67
Description:Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K.

Molecular Weight: 39.8kDa

Gene ID: 945

UniProt: P20138