Fibroblasts, Epithelial Cells antibody (PE)

Catalog No. ABIN319868

There are 2+ publications for this product available. The Mouse Monoclonal anti-Fibroblasts, Epithelial Cells antibody is suitable to detect Fibroblasts, Epithelial Cells in samples from Human. It has been validated for FACS.

Quick Overview for Fibroblasts, Epithelial Cells antibody (PE) (ABIN319868)

Target: Fibroblasts, Epithelial Cells

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: PE

Application: Flow Cytometry (FACS)

Clone: D7-FIB

Product Details

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Human foreskin Fibroblasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.25 mL (SM1214RT) or 1 mL (SM1214R) distilled water.

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA, 5 % Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

References

Raaijmakers, Widmer, Narechania, Eichhoff, Freiberger, Wenzina, Cheng, Mihic-Probst, Desalle, Dummer, Levesque: "Co-existence of BRAF and NRAS driver mutations in the same melanoma cells results in heterogeneity of targeted therapy resistance." in: Oncotarget, Vol. 7, Issue 47, pp. 77163-77174, (2018) (PubMed).

Nazareth, Broderick, Simpson-Abelson, Kelleher, Yokota, Bankert: "Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 9, pp. 5552-62, (2007) (PubMed).

Target Details

Target: Fibroblasts, Epithelial Cells

Alternative Name: Fibroplasts / Epithelial Cells

Background: A fibroblast is a connective-tissue cell of mesenchymal (somewhat undifferentiated) origin that secretes proteins from which the extracellular fibrillar matrix of connective tissue forms. Epithelial cells are cells that cover the surface of the body and line its cavities.Synonyms: Fibroblast Marker, Fibroblasten