Arachidonate 15-Lipoxygenase (ALOX15) antibody

Antigen: Arachidonate 15-Lipoxygenase (ALOX15)

Synonyms: 15LOX-1, 15-LOX-1, 12-LO, L-12LO, Alox12l, 12-LOX, Alox12, ALOX15, ALOX12, 15-LOX, DKFZp468J1413

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334342

Additional Information

Alternative name: ALOX15

Gene ID: 246

Immunogen: C-HYKTDVAVKDDPE

Description: All names and symbols: ALOX15, arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2. Official Symbol: ALOX15. RefSeq number(s): NP_001131.3

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Recommended concentration: 0.2-0.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334342 (0.2µg/ml) staining of nuclear HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: McDuffie, Maybee, Keller et al.: "Nonobese diabetic (NOD) mice congenic for a targeted deletion of 12/15-lipoxygenase are protected from autoimmune diabetes." in: Diabetes, Vol. 57, Issue 1, pp. 199-208, 2007 (PubMed).