ALOX15 antibody (AA 581-662)

Catalog No. ABIN2169710

Product Details anti-ALOX15 Antibody

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Binding Specificity: AA 581-662

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX15 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ALOX15/15 Lipoxygenase 1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ALOX15 antibody (ABIN2169710)

Francos-Quijorna, Amo-Aparicio, Martinez-Muriana, López-Vales: "IL-4 drives microglia and macrophages toward a phenotype conducive for tissue repair and functional recovery after spinal cord injury." in: Glia, Vol. 64, Issue 12, pp. 2079-2092, (2016) (PubMed).

Target Details for ALOX15

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Alternative Name: 15 Lipoxygenase 1 (ALOX15 Products)

Synonyms: 15-LOX-1 antibody, 15LOX-1 antibody, ALOX15 antibody, 12-LO antibody, Alox12l antibody, L-12LO antibody, 12-LOX antibody, Alox12 antibody, ALOX12 antibody, 15-LOX antibody, Alox12e antibody, arachidonate 15-lipoxygenase antibody, Arachidonate 15-lipoxygenase antibody, ALOX15 antibody, Alox15 antibody, Nmul_A0532 antibody, Sden_1680 antibody, Swoo_2919 antibody, PCC8801_3106 antibody, Cyan8802_3014 antibody, Nwat_1315 antibody

Background:

Synonyms: 12-LOX, 15LOX-1, 15-LOX-1, Arachidonate 15-lipoxygenase, 15-LOX, 12/15-lipoxygenase, Arachidonate 12-lipoxygenase, leukocyte-type, Arachidonate omega-6 lipoxygenase, ALOX15, LOG15

Background: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass.

Gene ID: 246

UniProt: P16050

Pathways: Regulation of Actin Filament Polymerization