Atonal Homolog 7 (Drosophila) (ATOH7) antibody

Antigen: Atonal Homolog 7 (Drosophila) (ATOH7)

Synonyms: Math5, bHLHa13, ath5, zath5, ATH5, ATOH7

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334354

Additional Information

Alternative name: Atoh7 (mouse)

Gene ID: 53404 (mouse), 365564 (rat)

Immunogen: CEQRGRDHPYLP

Description: All names and symbols: Atoh7, atonal homolog 7 (Drosophila), Math, atonal homolog 7. Official Symbol: Atoh7. RefSeq number(s): NP_058560.1

Specificity: Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave bands at approx 50kDa and 38kDa in Mouse Eye and Rat Optical Nerve lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 16.9kDa according to NP_058560.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Brown, Dagenais, Chen et al.: "Molecular characterization and mapping of ATOH7, a human atonal homolog with a predicted role in retinal ganglion cell development." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 13, Issue 2, pp. 95-101, 2002 (PubMed).