B and T Lymphocyte Associated (BTLA) antibody

Antigen: B and T Lymphocyte Associated (BTLA)

Synonyms: BTLA1, CD272, FLJ16065, MGC129743, MGC124217, MGC124218, A630002H24, BTLA

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334365

Additional Information

Alternative name: BTLA

Gene ID: 151888, 208154 (mouse), 407756 (rat)

Immunogen: KEAPTEYASICVRS

Description: All names and symbols: BTLA, B and T lymphocyte associated, BTLA1, CD272, FLJ16065, MGC129743, B and T lymphocyte attenuator. Official Symbol: BTLA. RefSeq number(s): NP_861445.3, NP_001078826.1

Specificity: This antibody is expected to recognize both reported isoforms (NP_861445.3, NP_001078826.1). Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx. 35kDa band observed in Mouse Spleen lysates (calculated MW of 32.8kDa according to Human NP_861445.3 and of 34.2kDa according to Mouse NP_001032808.2). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334365 (1µg/ml) staining of Mouse Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Lasaro, Tatsis, Hensley et al.: "Targeting of antigen to the herpesvirus entry mediator augments primary adaptive immune responses." in: Nature medicine, Vol. 14, Issue 2, pp. 205-12, 2008 (PubMed).