Carboxylesterase 1 (CES1) antibody

Antigen: Carboxylesterase 1 (CES1)

Synonyms: CEH, TGH, ACAT, CES2, HMSE, SES1, HMSE1, PCE-1, MGC117365, Ces-1, Ses-1, Es2, MGC109055, Ces1, MGC156521, CES1, CESDD1, CES-K1, LOC100009551, T2, MAT, THIL, SOAT, STAT, ACACT, ACAT1, RP11-215I23.2, ald, Acact, ACAT-1, AW550831, 8430426K15Rik, RATACAL, Acat-1, Acat, 6330585C21Rik, fd16h07, fd20g06, zgc:86832, wu:fd16h07, wu:fd20g06, MGC137917, MGC107795, acat1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334372

Additional Information

Alternative name: CES1

Gene ID: 1066

Immunogen: C-NTQAAQKLKDKE

Description: All names and symbols: CES1, carboxylesterase 1 (monocyte/macrophage serine esterase 1), ACAT, CEH, CES2, HMSE, HMSE1, MGC117365, PCE-1, SES1, TGH, acyl coenzyme A:cholesterol acyltransferase, carboxylesterase 1, carboxylesterase 2 (liver), cholesteryl ester hydrolase, egasyn, liver carboxylesterase, triacylglycerol hydrolase, monocyte/macrophage serine-esterase 1. Official Symbol: CES1. RefSeq number(s): NP_001020366.1, NP_001020365.1, NP_001257.4

Specificity: This antibody is expected to recognise all three reported isoforms (NP_001020366.1, NP_001020365.1, NP_001257.4). Human

Synonyms: CEH, TGH, ACAT, CES2, HMSE, SES1, HMSE1, PCE-1, MGC117365, Ces-1, Ses-1, Es2, MGC109055, Ces1, MGC156521, CES1, CESDD1, CES-K1, LOC100009551, T2, MAT, THIL, SOAT, STAT, ACACT, ACAT1, RP11-215I23.2, ald, Acact, ACAT-1, AW550831, 8430426K15Rik, RATACAL, Acat-1, Acat, 6330585C21Rik, fd16h07, fd20g06, zgc:86832, wu:fd16h07, wu:fd20g06, MGC137917, MGC107795, acat1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 55-60kDa band observed in Human Liver and Ileum lysates (calculated MW of 62.6kDa according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334372 (0.03µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing CES1 (RC220398) and probed with ABIN334372 (mock transfection in first lane), tested by Origene.

Publications

Publications: Steinberg, Kemp, Watt: "Adipocyte triglyceride lipase expression in human obesity." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 4, pp. E958-64, 2007 (PubMed).