Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4) antibody

Antigen: Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4)

Synonyms: EBN, BFNC, EBN1, NACHR, NACRA4, NACHRA4, FLJ95812, Acra4, ENFL1, Acra-4, NARAC, CHRNA4

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334374

Additional Information

Alternative name: CHRNA4

Gene ID: 1137

Immunogen: C-RASSHVETRAHAE

Description: All names and symbols: CHRNA4, cholinergic receptor, nicotinic, alpha 4, BFNC, EBN, EBN1, NACRA4, cholinergic receptor, nicotinic, alpha 4 subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, neuronal acetylcholine receptor alpha 4 subunit, neuronal nicotinic acetylcholine receptor alpha-4 subunit. Official Symbol: CHRNA4. RefSeq number(s): NP_000735.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments in Human Brain (Cerebral Cortex, Cerebellum and Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Bertrand, Picard, Le Hellard et al.: "How mutations in the nAChRs can cause ADNFLE epilepsy." in: Epilepsia, Vol. 43 Suppl 5, pp. 112-22, 2002 (PubMed).