Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4) (AA 29-43) antibody

Antigen: Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4)

Synonyms: EBN, BFNC, EBN1, NACHR, NACRA4, NACHRA4, FLJ95812, Acra4, ENFL1, Acra-4, NARAC, CHRNA4

Epitope: AA 29-43

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334375

Additional Information

Alternative name: CHRNA4 (aa29-43)

Gene ID: 1137, 11438 (mouse), 25590 (rat)

Immunogen: C-HVETRAHAEERLLKK

Description: All names and symbols: CHRNA4, cholinergic receptor, nicotinic, alpha 4, BFNC, EBN, EBN1, NACRA4,cholinergic receptor, nicotinic, alpha 4 subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, neuronal acetylcholine receptor alpha 4 subunit, neuronal nicotinic acetylcholine receptor alpha-4 subunit. Official Symbol: CHRNA4. RefSeq number(s): NP_000735.1

Specificity: This antibody is expected NOT to cross-react with the similar alpha 2 subunit. Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334375 (0.3µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Schwarz, Schwarz, Dorigo et al.: "Enhanced expression of hypersensitive alpha4* nAChR in adult mice increases the loss of midbrain dopaminergic neurons." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 7, pp. 935-46, 2006 (PubMed).