Early Growth Response 2 (EGR2) antibody

Antigen: Early Growth Response 2 (EGR2)

Synonyms: AT591, CMT1D, CMT4E, KROX20, FLJ14547, DKFZp686J1957, Egr-2, Zfp-6, Krox20, NGF1-B, Zfp-25, Krox-20

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334389

Additional Information

Alternative name: EGR2

Gene ID: 1959, 13654 (mouse), 114090 (rat)

Immunogen: HGTAGPDRKPFPC

Description: All names and symbols: EGR2, early growth response 2 (Krox-20 homolog, Drosophila) , CMT1D, CMT4E, DKFZp686J1957, FLJ14547, KROX20, KROX-20, Drosophila, homolog (early growth response-2), Krox-20 homolog, OTTHUMP00000062133 , early growth response 2 protein. Official Symbol: EGR2. RefSeq number(s): NP_000390.2

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 55kDa band observed in lysates of cell line HepG2 (calculated MW of 50.3kDa according to NP_000390.2). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

ABIN334389 (0.03µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: LeBlanc, Ward, Svaren: "Neuropathy-associated Egr2 mutants disrupt cooperative activation of myelin protein zero by Egr2 and Sox10." in: Molecular and cellular biology, Vol. 27, Issue 9, pp. 3521-9, 2007 (PubMed).