Fascin Homolog 2, Actin-Bundling Protein, Retinal (Strongylocentrotus Purpuratus) (FSCN2) antibody

Antigen: Fascin Homolog 2, Actin-Bundling Protein, Retinal (Strongylocentrotus Purpuratus) (FSCN2)

Synonyms: RFSN, RP30

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334397

Additional Information

Alternative name: Fascin 2

Gene ID: 25794, 238021 (mouse), 303741 (rat)

Immunogen: CHHRGSNQLDTNR

Description: All names and symbols: fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus), RFSN, RP30, fascin 2, retinal fascin,. Official Symbol: FSCN2 . RefSeq number(s): NP_001070650.1, NP_036550.1

Specificity: This antibody is expected to recognize both reported isoforms (NP_001070650.1 and NP_036550.1). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave bands at approx 75kDa and 22kDa in Mouse Eye lysates after 0.05µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 57.4kDa according to NP_001070650.1 and 55.1kDa according to NP_036550.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Neurology, Cytoskeleton, Microfilaments

Restrictions: For Research Use only

Publications

Publications: Vignjevic, Kojima, Aratyn et al.: "Role of fascin in filopodial protrusion." in: The Journal of cell biology, Vol. 174, Issue 6, pp. 863-75, 2006 (PubMed).