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Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) antibody

Antigen

Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1)

Synonyms GAD, SCP, FLJ45882, EP10, GAD25, GAD44, GAD67, Gad-1, Gad67, gad1, gad1-A, GAD1, GAD-67, MGC142602, GLUTAMATE DECARBOXYLASE, GLUTAMATE DECARBOXYLASE 1, MKP11.30, MKP11_30, DKFZp459M0324
Clonality Polyclonal
Host
Reactivity
Alternatives

Mouse (Murine)

Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA, Western Blotting (WB)
1 reference available
Catalog no. ABIN334405
Quantity 100 ug
Price 374.27 $   Plus shipping costs $45.00
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Additional Information

Alternative name GAD1 (GAD67)
Gene ID 2571, 14415 (mouse), 24379 (rat)
Immunogen C-PDSPQRREKLHK
Description All names and symbols: GAD1, glutamate decarboxylase 1 (brain, 67kDa), FLJ45882, GAD, SCP, OTTHUMP00000041055, glutamate decarboxylase 1, glutamate decarboxylase 1 (brain, 67kD), GAD67, GAD25. Official Symbol: GAD1. RefSeq number(s): NP_000808.2
Specificity This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2. Human, Mouse, Rat, Dog, Cow, Pig

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx. 70kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 66.9kDa according to Human NP_000808.2 and 66.6kDa according to Mouse NP_032103.2). Recommended concentration: 0.3-1µg/ml.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications

Huang, Akbarian: "GAD1 mRNA expression and DNA methylation in prefrontal cortex of subjects with schizophrenia." in: PLoS ONE, Vol. 2, Issue 8, pp. e809, 2007 (PubMed).

Alternatives

Alternatives for antigen "Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1)", type "Antibodies"
Hosts Rabbit (48), Goat (7), Mouse (7), Chicken (3)
Reactivities Human (57), Mouse (Murine) (41), Rat (Rattus) (40), Dog (Canine) (6), Cow (Bovine) (5), Pig (Porcine) (4), Cat (Feline) (2), Chicken (1), Monkey (1)
Applications Western Blotting (WB) (45), ELISA (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Immunofluorescence (IF) (18), Immunohistochemistry (IHC) (13), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes N-Term (7), C-Term (4), Transcript Variant GAD67 (3), Internal Region (2), AA 1-100 (1), AA 1-585 (1), AA 2-97 (1), Transcript Variant GAD25 (1)