Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) antibody
| Antigen | Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) |
| Synonyms | GAD, SCP, FLJ45882, EP10, GAD25, GAD44, GAD67, Gad-1, Gad67, gad1, gad1-A, GAD1, GAD-67, MGC142602, GLUTAMATE DECARBOXYLASE, GLUTAMATE DECARBOXYLASE 1, MKP11.30, MKP11_30, DKFZp459M0324 |
| Clonality | Polyclonal |
| Host |
Alternatives Goat |
| Reactivity |
Alternatives Mouse (Murine) |
| Conjugate |
Alternatives Un-conjugated |
| Application |
Alternatives ELISA, Western Blotting (WB) |
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1 reference available |
| Catalog no. | ABIN334405 |
| Quantity | 100 ug |
| Price | 374.27 $ Plus shipping costs $45.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | GAD1 (GAD67) |
| Gene ID | 2571, 14415 (mouse), 24379 (rat) |
| Immunogen | C-PDSPQRREKLHK |
| Description | All names and symbols: GAD1, glutamate decarboxylase 1 (brain, 67kDa), FLJ45882, GAD, SCP, OTTHUMP00000041055, glutamate decarboxylase 1, glutamate decarboxylase 1 (brain, 67kD), GAD67, GAD25. Official Symbol: GAD1. RefSeq number(s): NP_000808.2 |
| Specificity | This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2. Human, Mouse, Rat, Dog, Cow, Pig |
Application Details
| Application Notes | Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx. 70kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 66.9kDa according to Human NP_000808.2 and 66.6kDa according to Mouse NP_032103.2). Recommended concentration: 0.3-1µg/ml. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Restrictions | For Research Use only |
Images
| ABIN334405 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
Publications
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Huang, Akbarian: "GAD1 mRNA expression and DNA methylation in prefrontal cortex of subjects with schizophrenia." in: PLoS ONE, Vol. 2, Issue 8, pp. e809, 2007 (PubMed).
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Alternatives
Alternatives for antigen "Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1)", type "Antibodies"
| Hosts | Rabbit (48), Goat (7), Mouse (7), Chicken (3) |
| Reactivities | Human (57), Mouse (Murine) (41), Rat (Rattus) (40), Dog (Canine) (6), Cow (Bovine) (5), Pig (Porcine) (4), Cat (Feline) (2), Chicken (1), Monkey (1) |
| Applications | Western Blotting (WB) (45), ELISA (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Immunofluorescence (IF) (18), Immunohistochemistry (IHC) (13), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1) |
| Conjugates | Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1) |
| Epitopes | N-Term (7), C-Term (4), Transcript Variant GAD67 (3), Internal Region (2), AA 1-100 (1), AA 1-585 (1), AA 2-97 (1), Transcript Variant GAD25 (1) |




Alternatives