Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2D (GRIN2D) antibody

Antigen: Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2D (GRIN2D)

Synonyms: EB11, NR2D, NMDAR2D, nr2d

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334413

Additional Information

Alternative name: GRIN2D

Gene ID: 2906, 14814 (mouse), 24412 (rat)

Immunogen: C-TRRGSAHFSSLESE

Description: All names and symbols: GRIN2D, glutamate receptor, ionotropic, N-methyl D-aspartate 2D, EB11, NMDAR2D, N-methyl-D-aspartate receptor subunit 2D, estrogen receptor binding CpG island. Official Symbol: GRIN2D . RefSeq number(s): NP_000827.2

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 80kDa band in Human Brain (Cerebellum) and in Mouse Brain and Rat Brain lysates after 0.02µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 144kDa according to NP_000827.2. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Clarke, Johnson: "NMDA receptor NR2 subunit dependence of the slow component of magnesium unblock." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 21, pp. 5825-34, 2006 (PubMed).