Chromosome 18 Open Reading Frame 8 (C18orf8) antibody

Antigen: Chromosome 18 Open Reading Frame 8 (C18orf8)

Synonyms: C18orf8, MGC105615

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334435

Additional Information

Alternative name: MIC1 / C18orf8

Gene ID: 29919, 76482 (mouse), 291784 (rat)

Immunogen: C-DKLNHEYKKYLDAEQ

Description: All names and symbols: C18orf8, chromosome 18 open reading frame 8 , FLJ23453, HsT2591, MIC1 , colon cancer associated protein Mic1, colon cancer-associated protein Mic1. Official Symbol: C18orf8. RefSeq number(s): NP_037458.3

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 90kDa band in Human Bone Marrow, Spleen and Tonsil lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 75kDa according to NP_037458.3. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? An additional band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).