MutS Homolog 4 (E. Coli) (MSH4) (internal) antibody

Antigen: MutS Homolog 4 (E. Coli) (MSH4)

Synonyms: mMsh4, AV144863, 4930485C04Rik, MSH4

Epitope: internal

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334438

Additional Information

Alternative name: MSH4 (internal)

Gene ID: 4438, 55993 (mouse)

Immunogen: C-ETNSSTFMKEMKE

Description: All names and symbols: MSH4, mutS homolog 4 (E. coli), RP11-262K24.2, mutS homolog 4. Official Symbol: MSH4 . RefSeq number(s): NP_002431.2

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave bands at approx 60kDa and 35kDa in Human Testis, Ovary and Uterus lysates and in Mouse and Rat Testis lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 105kDa according to NP_002431.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Neyton, Lespinasse, Lahaye et al.: "CRM1-dependent nuclear export and dimerization with hMSH5 contribute to the regulation of hMSH4 subcellular localization." in: Experimental cell research, Vol. 313, Issue 17, pp. 3680-93, 2007 (PubMed).