NEDD1 antibody (Internal Region)

Catalog No. ABIN334442

The Goat Polyclonal anti-NEDD1 antibody is suitable to detect NEDD1 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for NEDD1 antibody (Internal Region) (ABIN334442)

Target: NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NEDD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-NEDD1 Antibody

Binding Specificity: Internal Region

Purpose: NEDD1

Sequence: NQTRNSEKFE KPEN

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NQTRNSEKFEKPEN, from the internal region of the protein sequence according to NP_690869.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in lysates of cell line HeLa (calculated MW of 72.0 kDa according to NP_690869.1). Recommended concentration: 1-3 μg/mL. An additional band was also consistently observed at 37 kDa. This band was successfully blocke

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NEDD1

Target: NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))

Alternative Name: NEDD1

Background: NEDD1, neural precursor cell expressed, developmentally down-regulated 1

Gene ID: 121441

NCBI Accession: NP_690869

Pathways: M Phase