Neuro-Oncological Ventral Antigen 1 (NOVA1) antibody

Antigen: Neuro-Oncological Ventral Antigen 1 (NOVA1)

Synonyms: Nova-1, MGC84002, NOVA1, si:ch211-222c22.9, G630039L02, 9430099M15Rik, nova1, MGC82481

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334449

Additional Information

Alternative name: NOVA1

Gene ID: 4857

Immunogen: C-REMPQNVAKTEPVS

Description: All names and symbols: NOVA1, neuro-oncological ventral antigen 1, Nova-1, paraneoplastic Ri antigen, ventral neuron-specific protein 1. Official Symbol: NOVA1. RefSeq number(s): NP_002506.2 , NP_006480.2 , NP_006482.1

Specificity: This antibody is expected to recognise all three reported isoforms (NP_002506.2 , NP_006480.2 , NP_006482.1) Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 50-55kDa band observed in Human Breast Cancer lysates (calculated MW of 51.7kDa according to NP_002506.2). In transfected HEK293 transiently expressing NOVA1 a band of approx. 52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334449 (0.01µg/ml) staining of Human Breast Cancer lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing NOVA1 (RC210407) and probed with ABIN334449 (mock transfection in first lane), tested by Origene.

Publications

Publications: Jelen, Ule, Zivin et al.: "Evolution of Nova-dependent splicing regulation in the brain." in: PLoS genetics, Vol. 3, Issue 10, pp. 1838-47, 2007 (PubMed).