ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) antibody

Antigen: ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1)

Synonyms: ORAT1, CRACM1, FLJ14466, TMEM142A, orai-1, Tmem142a, D730049H07Rik, RGD1311873, tmem142a, im:7146264, zgc:109721, orai2, MGC68830, ORAI1, MGC159526, LOC100218031

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334452

Additional Information

Alternative name: ORAI1 / CRACM1

Gene ID: 84876, 109305 (mouse)

Immunogen: C-KKQPGQPRPTSKP

Description: All names and symbols: ORAI1, CRACM1, ORAI calcium release-activated calcium modulator 1, FLJ14466, ORAT1, TMEM142A, calcium release-activated calcium channel protein 1, calcium release-activated calcium modulator 1, transmembrane protein 142A. Official Symbol: ORAI1. RefSeq number(s): NP_116179.2

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx. 50-55kDa band observed in Human Liver and Human Placenta lysates (calculated MW of 32.7kDa according to NP_116179.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Li et al, ,J Biol Chem. 2007 Oct 5,282(40):29448-56., PMID: 17702753). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334452 (0.03µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Vig, DeHaven, Bird et al.: "Defective mast cell effector functions in mice lacking the CRACM1 pore subunit of store-operated calcium release-activated calcium channels." in: Nature immunology, Vol. 9, Issue 1, pp. 89-96, 2007 (PubMed).