Purinergic Receptor P2X, Lig-Nd-Gated Ion Channel, 4 (P2RX4) antibody

Antigen: Purinergic Receptor P2X, Lig-Nd-Gated Ion Channel, 4 (P2RX4)

Synonyms: P2X4, P2X4R

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334455

Additional Information

Alternative name: P2X4 / P2X4R

Gene ID: 5025, 18438 (mouse), 29659 (rat)

Immunogen: C-YREKKYKYVEDYEQ

Description: All names and symbols: P2X4, P2X4R, P2RX4, purinergic receptor P2X, ligand-gated ion channel, 4, ATP receptor, ATP-gated cation channel protein, P2X purinoceptor 4, P2X receptor, subunit 4, purinergic receptor P2X ligand-gated ion channel 4, purinergic receptor P2X4, purinoceptor P2X4. Official Symbol: P2RX4. RefSeq number(s): NP_002551.2

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 60kDa band observed in Human Kidney lysates (calculated MW of 43.4kDa according to NP_002551.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Brown et al, J Biol Chem. 2004 Sep 17,279(38):39485-94., PMID: 15262999). Recommended concentration: 0.3-1µg/ml. Size: 100μg specific antibody in 200μl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334455 (0.3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Yeung, Kharidia, Brown et al.: "Enhanced expression of the P2X4 receptor in Duchenne muscular dystrophy correlates with macrophage invasion." in: Neurobiology of disease, Vol. 15, Issue 2, pp. 212-20, 2004 (PubMed).