P2RX4 antibody (Internal Region)
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- Target See all P2RX4 Antibodies
- P2RX4 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This P2RX4 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
- Purpose
- P2rx4 (mouse)
- Sequence
- ETDSVVSSVT TKAK
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-ETDSVVSSVTTKAK, from the internal region of the protein sequence according to NP_035156.2.
- Isotype
- IgG
- Top Product
- Discover our top product P2RX4 Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 5 μg/mL.
Western Blot: Approx 48-50 kDa band observed in lysates of cell line A549 (calculated MW of 43.4 kDa according to Human NP_002551.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1
Peptide ELISA: antibody detection limit dilution 1:16000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- P2RX4 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4))
- Alternative Name
- P2rx4 (P2RX4 Products)
- Synonyms
- P2X4 antibody, P2X4R antibody, AI504491 antibody, AW555605 antibody, D5Ertd444e antibody, p2rx4 antibody, p2x4 antibody, wu:fi03f02 antibody, p2x4r antibody, p2rx4.2 antibody, p2xr4.2 antibody, purinergic receptor P2X 4 antibody, purinergic receptor P2X, ligand-gated ion channel 4 antibody, purinergic receptor P2X, ligand-gated ion channel, 4a antibody, purinergic receptor P2X, ligand gated ion channel, 4 L homeolog antibody, purinergic receptor P2X, ligand-gated ion channel, 4b antibody, P2RX4 antibody, P2rx4 antibody, p2rx4a antibody, p2rx4.L antibody, p2rx4b antibody
- Background
- P2rx4, purinergic receptor P2X, ligand-gated ion channel, 4, P2X4, AI504491, AW555605, D5Ertd444e, ATP receptor, OTTMUSP00000023741, OTTMUSP00000023745, OTTMUSP00000023746, OTTMUSP00000023747, P2X purinoceptor 4, ionotropic purinergic receptor
- Gene ID
- 5025, 18438, 29659
- NCBI Accession
- NP_035156
- Pathways
- Synaptic Membrane
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