Poly(rC) Binding Protein 4 (PCBP4) antibody

Antigen: Poly(rC) Binding Protein 4 (PCBP4)

Synonyms: LIP4, MCG10, AlphaCP-4, 1200003L19Rik, Pcbp4, zgc:77347, wu:fc68f03, PCBP4, MGC140586

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334457

Additional Information

Alternative name: PCBP4

Gene ID: 57060, 59092 (mouse), 363133 (rat)

Immunogen: C-NGSKKAERQKFSPY

Description: All names and symbols: PCBP4, poly(rC) binding protein 4 , LIP4, MCG10 , LYST-interacting protein, RNA binding protein MCG10, alphaCP-4 protein, poly(rC)-binding protein 4, alpha CP-4,. Official Symbol: PCBP4. RefSeq number(s): NP_065151.1, NP_127501.1, NP_127502.1, NP_127503.1

Specificity: This antibody is expected to recognise all reported isoforms (NP_065151.1, NP_127501.1, NP_127502.1 and NP_127503.1). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx. 40kDa band observed in lysates of cell line HeLa (calculated MW of 41.4kDa according to NP_127502.1 and NP_127503.1). In transfected HEK293 transiently expressing PCBP4 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334457 (0.03µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing PCBP4 (RC200749) and probed with ABIN334457 (mock transfection in first lane), tested by Origene.

Publications

Publications: Pio, Zudaire, Pino et al.: "Alpha CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant alpha CP-4a, is underexpressed in lung cancer." in: Cancer research, Vol. 64, Issue 12, pp. 4171-9, 2004 (PubMed).