RPL8 antibody (Internal Region)

Catalog No. ABIN334478

The Goat Polyclonal anti-RPL8 antibody has been validated for WB, ELISA and IHC. It is suitable to detect RPL8 in samples from Human.

Quick Overview for RPL8 antibody (Internal Region) (ABIN334478)

Target: RPL8 (Ribosomal Protein L8 (RPL8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RPL8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-RPL8 Antibody

Binding Specificity: Internal Region

Purpose: ribosomal protein L8

Sequence: KAGRAYHKYK AKRNC

Specificity: The reported variants represent identical protein (NP_000964.1 and NP_150644.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KAGRAYHKYKAKRNC, from the internal region of the protein sequence according to NP_000964.1, NP_150644.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Cerebral Cortex and Spleen. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 28-30 kDa band observed in lysates of cell line HepG2 (calculated MW of 28.0 kDa according to NP_000964.1 and NP_150644.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RPL8

Target: RPL8 (Ribosomal Protein L8 (RPL8))

Alternative Name: RPL8

Background: Ribosomal protein L8, 60S ribosomal protein L8

Gene ID: 6132, 26961, 26962

NCBI Accession: NP_000964

Pathways: Regulation of Cell Size