SAR1 Homolog B (S. Cerevisiae) (SAR1B) antibody

Antigen: SAR1 Homolog B (S. Cerevisiae) (SAR1B)

Synonyms: ANDD, CMRD, GTBPB, SARA2, Sara2, SARA1B, SAR1B, MGC127165

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334486

Additional Information

Alternative name: SAR1B / SARA2

Gene ID: 51128, 66397 (mouse), 287276 (rat)

Immunogen: C-DHERLLESKEELD

Description: All names and symbols: SAR1B, SARA2, SAR1 gene homolog B (S. cerevisiae), CMRD, GTBPB, 2310075M17Rik, GTP-binding protein Sara, SAR1a gene homolog 2. Official Symbol: SAR1B . RefSeq number(s): NP_001028675.1, NP_057187.1

Specificity: Both reported variants (NP_001028675.1 and NP_057187.1) represent identical protein Human, Mouse, Rat,

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx. 25kDa band observed in Mouse Liver and Rat Liver lysates (calculated MW of 22.4kDa according to human NP_057187.1 and mouse NP_079811.1). In transfected HEK293 transiently expressing SAR1B a band of approx. 26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334486 (0.03µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing SAR1B (RC210593) and probed with ABIN334486 (mock transfection in first lane), tested by Origene.

Publications

Publications: Evans, Burdge, Wootton et al.: "Tissue-specific stable isotope measurements of postprandial lipid metabolism in familial combined hyperlipidaemia." in: Atherosclerosis, Vol. 197, Issue 1, pp. 164-70, 2008 (PubMed).