SID1 Transmembrane Family, Member 1 (SIDT1) antibody

Antigen: SID1 Transmembrane Family, Member 1 (SIDT1)

Synonyms: SID1, SID-1, FLJ20174, B830021E24Rik, AW045928

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334490

Additional Information

Alternative name: SIDT1

Gene ID: 54847

Immunogen: C-QEKENQTWNLQRKKN

Description: All names and symbols: SIDT1, SID1 transmembrane family, member 1, B830021E24Rik, FLJ20174, SID-1, SID1. Official Symbol: SIDT1. RefSeq number(s): NP_060169.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 50kDa band in Human Brain (Cerebellum) and Human Breast lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 93.8kDa according to NP_060169.2. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Duxbury, Ashley, Whang: "RNA interference: a mammalian SID-1 homologue enhances siRNA uptake and gene silencing efficacy in human cells." in: Biochemical and biophysical research communications, Vol. 331, Issue 2, pp. 459-63, 2005 (PubMed).