STEAP Family Member 4 (STEAP4) antibody

Antigen: STEAP Family Member 4 (STEAP4)

Synonyms: TIARP, STAMP2, TNFAIP9, FLJ23153, DKFZp666D049, MGC68826, STEAP4, MGC108044, steap4

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334498

Additional Information

Alternative name: STEAP4 / Dudulin4

Gene ID: 79689, 117167 (mouse)

Immunogen: CVDNTLTRIRQGWERN

Description: All names and symbols: STEAP family member 4, DKFZp666D049 , FLJ23153 , STAMP2 , TIARP , TNFAIP9, six transmembrane prostate protein 2 , tumor necrosis factor, alpha-induced protein 9 , tumor necrosis-alpha-induced adipose-related protein. Official Symbol: STEAP4. RefSeq number(s): NP_078912.2

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx. 50-55kDa band observed in Human Adipose lysates (calculated MW of 52.0kDa according to NP_078912.2). In transfected HEK293 transiently expressing Dudulin4 a band of approx. 52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334498 (0.3µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Dudulin4 (RC216917) and probed with ABIN334498 (mock transfection in first lane), tested by Origene.

Publications

Publications: Wellen, Fucho, Gregor et al.: "Coordinated regulation of nutrient and inflammatory responses by STAMP2 is essential for metabolic homeostasis." in: Cell, Vol. 129, Issue 3, pp. 537-48, 2007 (PubMed).