ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8) antibody

Antigen: ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8)

Synonyms: HI, SUR, HHF1, MRP8, PHHI, SUR1, ABC36, HRINS, TNDM2, Sur, Sur1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334499

Additional Information

Alternative name: SUR1 / ABCC8

Gene ID: 6833, 20927 (mouse), 25559 (rat)

Immunogen: C-EFDKPEKLLSRKD

Description: All names and symbols: ABCC8, SUR1, ATP-binding cassette, sub-family C (CFTR/MRP), member 8, ABC36, HHF1, HI, HRINS, MRP8, PHHI, SUR, TNDM2, ATP-binding cassette, sub-family C, member 8, sulfonylurea receptor (hyperinsulinemia). Official Symbol: ABCC8. RefSeq number(s): NP_000343.2

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 170kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 177kDa according to NP_000343.2). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 23kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334499 (0.5µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: de Wet, Rees, Shimomura et al.: "Increased ATPase activity produced by mutations at arginine-1380 in nucleotide-binding domain 2 of ABCC8 causes neonatal diabetes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 48, pp. 18988-92, 2007 (PubMed).