U2 Small Nuclear RNA Auxiliary Factor 1-Like 4 (U2AF1L4) antibody

Antigen: U2 Small Nuclear RNA Auxiliary Factor 1-Like 4 (U2AF1L4)

Synonyms: U2AF1L4, FLJ22573, U2af26, U2AF1L3, FLJ35525, MGC33901, U2AF1RS3, U2AF1-RS3, U2AF1L3V1, AA407033, AF419339, AI451269, AW553050, TMEM149, MGC127195, MGC30332, F630003I17Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334521

Additional Information

Alternative name: U2AF1L4

Gene ID: 199746, 233073 (mouse), 361542 (rat)

Immunogen: C-EDGERAVAELSNR

Description: All names and symbols: U2AF1L4, U2 small nuclear RNA auxiliary factor 1-like 4, FLJ35525, MGC33901, U2AF1-RS3, U2AF1L3, U2AF1L3V1, U2AF1RS3, U2af26, U2 small nuclear RNA auxiliary factor 1-like 3, U2(RNU2) small nuclear RNA auxiliary factor 1-like 3. Official Symbol: U2AF1L4. RefSeq number(s): NP_001035515.1 , NP_659424.2

Specificity: This antibody is expected to recognise both reported isoforms (NP_001035515.1 and NP_659424.2). No cross-reactivity with U2AF1 expected. Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 20Da band observed in Human Brain (Cerebellum) and Lung lysates (calculated MW of 21.3kDa according to NP_001035515.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334521 (0.1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Chen, Ji, Dou et al.: "Cloning and characterization of a novel splice variant of human U2AF1L3 gene." in: DNA sequence : the journal of DNA sequencing and mapping, Vol. 17, Issue 4, pp. 282-6, 2007 (PubMed).